A Colorimetric Method for Monitoring Tryptic Digestion Prior to Shotgun Proteomics
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چکیده
منابع مشابه
A Colorimetric Method for Monitoring Tryptic Digestion Prior to Shotgun Proteomics
Tryptic digestion is an important preanalytical step in shotgun proteomics because inadequate or excessive digestion can result in a failed or incomplete experiment. Unfortunately, this step is not routinely monitored before mass spectrometry because methods available for protein digestion monitoring either are time/sample consuming or require expensive equipment. To determine if a colorimetric...
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MOTIVATION One of the major problems in shotgun proteomics is the low peptide coverage when analyzing complex protein samples. Identifying more peptides, e.g. non-tryptic peptides, may increase the peptide coverage and improve protein identification and/or quantification that are based on the peptide identification results. Searching for all potential non-tryptic peptides is, however, time cons...
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Here, we describe the novel use of a volatile surfactant, perfluorooctanoic acid (PFOA), for shotgun proteomics. PFOA was found to solubilize membrane proteins as effectively as sodium dodecyl sulfate (SDS). PFOA concentrations up to 0.5% (w/v) did not significantly inhibit trypsin activity. The unique features of PFOA allowed us to develop a single-tube shotgun proteomics method that used all ...
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The peptides produced by the tryptic digestion of S-aminoethylated bovine pancreatic ribonuclease support the generally accepted amino acid sequence for this enzyme and also demonstrate the usefulness as well as some special limitations of the aminoethylation procedure. The relative extents of hydrolysis at different peptide bonds were estimated from the yields of purified peptides. Under the c...
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ژورنال
عنوان ژورنال: International Journal of Proteomics
سال: 2014
ISSN: 2090-2166,2090-2174
DOI: 10.1155/2014/125482